Figure 7
Localization of endocytic vesicles by FM4-64 staining in tobacco zygotes and proembryos. (A 1 -H 1 ) The fluorescence images of living zygotes and proembryos stained by FM4-64. In a zygote, the fluorescence of FM4-64 evenly distributes in the whole cytoplasm (A 1 ). During cell plate formation, endocytic vesicles mainly localize in the new cell plate edges (B 1 -D 1 ). After treating with 50 μM β-GlcY, the fluorescence of FM4-64 evenly distributes in cytoplasm of in vitro cultured proembryos (E 1 and F 1 ). In proembryos cultured without β-GlcY, the fluorescence images are similar to that of in vivo proembryos (G 1 ). (A 2 -H 2 ) Bright-field images of zygotes and proembryos corresponding to A 1 -G 1 . (A 3 -H 3 ) Fluorescence images of DAPI-stained nuclei corresponding to bright-field images. Bars = 10 μm.