Figure 6
Distribution of cellulose revealed by Calcofluor white (CW) staining in zygotes and proembryos. (A 1 -D 1 ) In the isolated zygotes and proembryos in vivo, the fluorescence of CW deposited both in cell surface and cell plate walls. The cell plates are highlighted by arrows. (E 1 -F 1 ) With 50 μM β-GlcY treatment, the fluorescence in the newly formed cell plate walls weakens. (G 1 ) The fluorescence image of untreated proembryo cultured in vitro is similar with that of in vivo. (A 2 -G 2 ) Bright-field images of zygotes and proembryos corresponding toA 1 -G 1 . (A 3 -G 3 ) Fluorescence images of DAPI-stained nuclei corresponding to bright-field images. Bars = 10 μm.