Figure 1

Identification of apyrase double knockouts complemented with AtAPY1::AtAPY1-SNAP or 35S::AtAPY1-GFP. (A) Genomic DNA was isolated from DKO plants complemented with either AtAPY1::AtAPY1-SNAP (DKO-SNAP) or 35S::AtAPY1-GFP (DKO-GFP) and subjected to PCR analysis. The PCR products for AtAPY1 (1.0 kb), AtAPY2 (0.9 kb), the T-DNA null mutations apy1 (0.7 kb) and apy2 (0.6 kb), AtAPY1-SNAP (0.6 kb), AtAPY1-GFP (0.7 kb) and SPIK::AtAPY2 (0.4 kb) were analyzed by agarose gel electrophoresis. Genomic DNA from plants which had been tested positive for the respective amplification products before served as the positive controls (+). The negative PCR controls (−) were run without addition of any DNA template. (B) The phenotype of the WT, the DKO and the DKO rescued with either the SNAP- or GFP-tagged AtAPY1 are shown. The genetic backgrounds are represented by colored symbols. Images of 8-d- (WT), 14-d- (DKO), 7-d- (DKO-SNAP), 7-d-old (DKO-GFP) seedlings and of 30-d-old adult plants were taken. Scale bars equal 1 cm.