Figure 1

Flow diagram of smallRNA and degradome library analysis. Three smRNA libraries and three mRNA degradome libraries were made from whole barley caryopses at three developmental stages: A (1–5 DPA), B (6–10 DPA) and C (11–15 DPA). Light grey boxes indicate the input data (RE stands for Repeat Elements) and dark grey boxes represent the output data. The (1) known miRNAs, (2) new miRNAs and (3) 19-23nt smRNAs identified from the analysis of the smRNA libraries were then used in combination with the degradome libraries to find potential miRNA regulated genes.