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Figure 3 | BMC Plant Biology

Figure 3

From: Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea(L.) and their relevance for perfume manufacture

Figure 3

GC-MS analysis of Ss LPPS reaction products. Shown are total ion chromatograms (TIC) of [A] reaction products obtained from in vitro assays with Ni2+-affinity-purified SsLPPS using GGPP 1 as a substrate and subsequent dephosphorylation, [B] in vitro assay products without dephosphorylation, [C] dephosphorylated GGPP, and [D] authentic sclareol standard (Sigma). GC-MS analysis was performed on an Agilent HP5ms column with electronic impact ionization at 70 eV. Results were confirmed with three independent experiments. Identification of reaction products was achieved by comparison to authentic standards or reference mass spectra from the National Institute of Standards and Technology MS library searches (Wiley W9N08L): peak a, epi-manoyl oxide 8; peak b, manoyl oxide 7; peak c, putative 13(16),-14-labdien-8-ol; peak d, putative copalol; peak e, sclareol 4; peak f, unknown compound; peak g, labda-13-en-8,15-diol; peak h, geranylgeraniol.

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BMC Plant Biology

ISSN: 1471-2229

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