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Figure 5 | BMC Plant Biology

Figure 5

From: Characterization of a putative grapevine Zn transporter, VvZIP3, suggests its involvement in early reproductive development in Vitis vinifera L

Figure 5

Complementation of the ZHY3 (zrt1zrt2Δ) yeast mutant with VvZIP3. The double mutant strain was transformed with either the empty vector pYES2 or with the pYES2-VvZIP3 construction. The wild type parental strain DY1457 (WT) was also transformed with pYES2 as a control. The transformed cells were adjusted to OD600 of 1 and 5 μl of serial dilutions (from left to right in each panel) and were spotted on SC-U plates supplemented with different concentrations of ZnCl2. Plates were incubated for 6 days at 30°C.

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