Figure 5From: Characterization of a putative grapevine Zn transporter, VvZIP3, suggests its involvement in early reproductive development in Vitis vinifera LComplementation of the ZHY3 (zrt1zrt2Δ) yeast mutant with VvZIP3. The double mutant strain was transformed with either the empty vector pYES2 or with the pYES2-VvZIP3 construction. The wild type parental strain DY1457 (WT) was also transformed with pYES2 as a control. The transformed cells were adjusted to OD600 of 1 and 5 μl of serial dilutions (from left to right in each panel) and were spotted on SC-U plates supplemented with different concentrations of ZnCl2. Plates were incubated for 6 days at 30°C.Back to article page