Complementation of the ZHY3 (zrt1zrt2Δ) yeast mutant with VvZIP3. The double mutant strain was transformed with either the empty vector pYES2 or with the pYES2-VvZIP3 construction. The wild type parental strain DY1457 (WT) was also transformed with pYES2 as a control. The transformed cells were adjusted to OD600 of 1 and 5 μl of serial dilutions (from left to right in each panel) and were spotted on SC-U plates supplemented with different concentrations of ZnCl2. Plates were incubated for 6 days at 30°C.