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Table 2 Effect of the chromosome doubling procedure on plant fertility

From: Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Colchicine treatment

Plants

Fertile plants [%]

Fertile plants per 100 embryos

Mean sterile tillers1

Mean fertile tillers2

Mean seeds per fertile tiller3

24 h - 0 mM

65

29.2 a

5.8 ab

0.0 a

5.6 cdef

29.1 bcde

24 h - 0.3 mM

47

27.7 a

5.4 ab

0.4 a

6.9 abcd

33.8 a

24 h - 1 mM

60

50.0 ab

12.5 b

0.5 a

4.5 fg

24.2 e

24 h - 5 mM

35

45.7 ab

8.9 ab

0.3 a

4.9 efg

24.1 cde

48 h - 0 mM

43

32.6 a

6.7 ab

0.7 a

7.7 ab

19.8 bcde

48 h - 0.3 mM

55

50.9 ab

11.7 b

0.9 a

7.0 abc

22.4 bcde

48 h - 1 mM

34

61.8 b

8.8 ab

0.5 a

5.7 defg

33.7 abcd

48 h - 5 mM

6

66.7 ab

3.3 a

0.3 a

7.0 a

26.6 ab

72 h - 0 mM

26

38.5 a

4.8 ab

0.9 a

6.3 abcde

32.0 ab

72 h - 0.3 mM

50

52.0 ab

10.8 ab

0.5 a

5.0 defg

31.3 bcde

72 h - 1 mM

32

59.4 ab

7.9 ab

0.9 b

6.5 abcde

27.4 abc

72 h - 5 mM

32

56.2 ab

6.7 ab

0.2 a

6.3 bcde

36.7 a

in vivo

153

52.3 ab

9.6 ab

2.9 b

3.7 g

31.0 de

  1. 1 mean number of sterile and 2 fertile tillers per seed-setting plant, and 3 mean number of seeds per fertile or partially fertile tiller. Means of columns followed by different letters indicate significant differences at the α = 0.05 level.