Table 1 Effect of in vitro versus in vivo colchicine treatment
Colchicine treatment | Transferred embryos | Regeneration rate | Green plants [%] | Survival rate [%] |
|---|---|---|---|---|
24 h - 0 mM | 330 | 42.4 a | 54.3 a | 85.5 a |
24 h - 0.3 mM | 240 | 39.2 a | 61.7 ab | 81.0 ab |
24 h - 1 mM | 240 | 41.7 a | 72.0 ab | 83.3 ab |
24 h - 5 mM | 180 | 41.7 a | 65.3 ab | 71.4 ab |
48 h - 0 mM | 210 | 43.3 a | 75.8 b | 62.3 bc |
48 h - 0.3 mM | 240 | 47.1 a | 59.3 ab | 82.1 ab |
48 h - 1 mM | 240 | 47.1 a | 69.0 ab | 43.6 cd |
48 h - 5 mM | 120 | 24.2 b | 41.4 a | 50.0 ab |
72 h - 0 mM | 210 | 42.4 a | 48.3 a | 60.5 b |
72 h - 0.3 mM | 240 | 43.8 a | 69.5 ab | 68.5 ab |
72 h - 1 mM | 240 | 33.3 a | 62.5 ab | 64.0 ab |
72 h - 5 mM | 270 | 26.3 b | 71.8 ab | 62.7 ab |
in vivo | 835 | 58.8 c | 70.9 b | 44.0 d |