Figure 2

Salinity and development regulated expression of OsMT1e-P in rice (Pokkali) grown in green house under standard agronomic practices. (A) Expression pattern of OsMT1e-P at various developmental stages of Oryza sativa based on microarray data (www.genevestigator.com). The expression data shown here is normalized, high quality and manually curated data obtained from several hundreds of independent experiments. The stage of development of plant is also shown diagrammatically in the figure. (B) Time course RNA gel blot analysis of OsMT1e-P transcripts in shoots of Pokkali rice seedlings stressed with 200 mM NaCl for 10′, 20′, 30′, 24 h, 48 h or 72 h. (C) Time course RNA gel blot analysis of OsMT1e-P transcripts in shoots of Pokkali rice tillers stressed with 200 mM NaCl for 30′ or 24 h. (D) RNA gel blot analysis of the OsMT1e-P expression profile in various tissues of mature rice plants (stressed with 200 mM NaCl for 24 h), grown in green house. The 0 h time point served as the control in all the analyses. The RNA gel blot analysis was performed twice with similar results. The EtBr stained RNA gel is shown below each RNA blot as loading control. The scanned intensity of each band is shown above the corresponding blot in the form of a histogram.