Table 1 Primers used in this study
Name | Sequence (5’-3’) | Comments |
|---|---|---|
NbTC9992-1F | GCTGTCAAAGAAGTGTCATTA | Specific primer for TC9992 |
NbTC9992-1280R | ACCGTTTATTAATCACTATATTGC | Specific primer for TC9992 |
NtBP1333-1 F | CTTAATGGGCAAGCAGCTAATC | Specific primer for BP133312 |
NtBP1333-447R | TCAAGATTGTATGTTGTCTGCTC | Specific primer for BP133312 |
LeBI9315-123F | GTTGCAATTAAACAAGTTTCTCTGGA | Specific primer for BI931567 |
LeBI9315-658R | GGCTGAAGATCATAGTACGG | Specific primer for BI931567 |
NbTC9992-458R-5RACE | GCTTGTCCATAGCCTTGGTTCTTCCT | 5’-RACE for TC9992 |
NbTC9992-49R-5RACE | TTTGCCTTCCCCCATCGCCTTGAT | 5’-RACE for TC9992 |
NbTC9992-F1 | GGATTGGAAAGGGGGAACCT | Sequencing |
NtMAP3Kb-566F | TCCGCCGGTCATGTCACT | Sequencing |
NtBP1333-199R-5RACE | ACATGGCTGCAGCTGCTTCATATTC | 5’-RACE for BP133312 |
NtBP1333-91R-nested | TACTATCCTTCTGCATAACTGACTGCAA | 5’-RACE for BP133312 |
NtBP1333-333F-5RACE | GAGAATCTCTCATCAGCCAGATGTTC | 3’-RACE for BP133312 |
NtBP1333-375F-nested | CAAACCTGTTGGTGGGGTACGAAT | 3’-RACE for BP133312 |
NtBP1333-F1 | AGACGCGCATAATTCGCATC | Sequencing |
NtBP1333-R1 | TGAGCTCTCGTTTGGTAATAAG | Sequencing |
LeBI9315-507R-5RACE | CATCTGCCTCTGTCAACTTTGTTGCA | 5’-RACE for LeBI931567 |
LeBI9315-165R-nested | CCTCCTGAGCAATATTCTCCAGAGA | 5’-RACE for LeBI931567 |
LeBI9315-482F-3RACE | TGCAACAAAGTTGACAGAGGCAGATG | 3’-RACE for LeBI931567 |
LeBI9315-564F-nested | ATGTCGGGAGTATGTGCTGCATCTG | 3’-RACE for LeBI931567 |
LeBI9315-F1 | CTGAGAAAGTTCTTGCAAACG | Sequencing |
LeBI9315-F2 | GAACAGATGAATCAGAAGATG | Sequencing |
LeBI9315-F3 | AAAGCACTCCATATAAACACAG | Sequencing |
LeBI9315-R1 | GGTATACATCAAGTCCACCAT | Sequencing |
LeBI9315-R2 | GAGGGAGTATGCTCTCATG | Sequencing |
Kp-NbMAPKKKa-1F | GGGGTACCGAATGCCTGCTTGGTGGGGAA | Full-length ORF of NbMAPKKKα |
Xh-NbMAPKKKa-1836R | GGCTCGAGTGCTAAAGAATTGGTCTTAGTTTTG | Full-length ORF of NbMAPKKKα |
Kp-NbMAPKKKb-1F | CCGGTACCGAATGCATCGATTGCCAGGAATTTTTGC | Full-length ORF of NbMAPKKKβ |
EcV-NbMAPKKKb-1695R | GGGATATCTTTAAAGCCTCTTGCCCAGATTTTG | Full-length ORF of NbMAPKKKβ |
Kp-NbMAPKKKg-1F | GGGGTACCGAATGCGTTGGTGGCAGAACG | Full-length ORF of NbMAPKKKγ |
Xh-NbMAPKKKg-1956R | GGCTCGAGTGCTACCTCTCTAGAGATAAACG | Full-length ORF of NbMAPKKKγ |
Kp-NbMAPKKKbkinase-F | CCGGTACCGAATGTACTGGGACAAAGGTGATCT | Kinase domain of NbMAPKKKβ |
EcV-NbMAPKKKbkinase-R | GGGATATCTTTACACAAAAGGATGCTCCAAGA | Kinase domain of NbMAPKKKβ |
Kp-NbMAPKKKgkinase-F | GGGGTACCGAATGTGGCAAAAAGGGAAGCTTATTG | Kinase domain of NbMAPKKKγ |
Xh-NbMAPKKKgkinase-R | GGCTCGAGTGTTACATAAATCGATGTTCCAATAAC | Kinase domain of NbMAPKKKγ |
Kp-NbMAPKKKekinase-F | GGGGTACCGAATGAAATATATGCTCGGAGATGAG | Kinase domain of NbMAPKKKε2 |
Xh-NbMAPKKKekinase-R | GGCTCGAGTGTTATATCCATGGATGTGAAAGTAG | Kinase domain of NbMAPKKKε2 |
NbMAPKKKb-K381M-F | TTTTGCTGTCATGGAAGTGTCATTACTTGATCA | K → M mutant of NbMAPKKKβ |
NbMAPKKKb-K381M-R | ATGACACTTCCATGACAGCAAAAAAGAAACCG | K → M mutant of NbMAPKKKβ |
NbMAPKKKg-K374M-F | CTGGAGCTTTATGTGCGATGATGGAAGTTGAATT ATTACCGGA | K → M mutant of NbMAPKKKγ |
NbMAPKKKg-K374M-R | TCCGGTAATAATTCAACTTCCATCATCGCACATAA AGCTCCAG | K → M mutant of NbMAPKKKγ |
NbMAPKKKa-255F | GGTTGTTTTGGGATGTGGGGTCAG | Real-time RT-PCR for NbMAPKKKα |
NbMAPKKKa-393R | CAGTGGGCTCAACCTATTATCGCC | Real-time RT-PCR for NbMAPKKKα |
NbMAPKKKb-1179F | CACAAGGCAGATTTTACATGGTTTG | Real-time RT-PCR for NbMAPKKKβ |
NbMAPKKKb-1286R | AGCTTGACCGATCCGTTAGCA | Real-time RT-PCR for NbMAPKKKβ |
NbMAPKKKg-903F | CCGTGAGTGTAGTGCTCAGGGTAA | Real-time RT-PCR for NbMAPKKKγ |
NbMAPKKKg-984R | TGCCGTAGGCTGCTGTGATG | Real-time RT-PCR for NbMAPKKKγ |
Nb18S-193F | ATACGTGCAACAAACCCCGAC | Real-time RT-PCR for Nb18S rRNA |
Nb18S-280R | TGAATCATCGCAGCAACGG | Real-time RT-PCR for Nb18S rRNA |