Characterization of fln insertion lines. (A) Representation of the FLN1 (At3g54090) and FLN2 (At1g69200) genes. T-DNA insertions are indicated with large, black triangles (not to scale) with allele designations above them. Exons, UTRs, introns, and deletions are indicated by black boxes, white boxes, lines, and a small triangle, respectively. Scale bars represent 100 bp. Red arrows indicate positions of primers used for RT-PCR. Graphics were generated with Exon-Intron Graphic Maker (http://wormweb.org/exonintron). (B) RT-PCR analysis of fln1 and fln2 alleles. 7-day old light grown seedlings were used for RNA extraction. The UBQ10 reactions indicate equivalent RNA inputs for Col-0 and mutant lines, and minus (−) RT reactions are samples treated equivalently except reverse transcriptase was not included in the RT reaction. Experiments are representative of 3 biological replicates. (C) 7-day old phenotype of fln mutants. Seeds segregating for fln alleles grown under a 16 h photoperiod at 18o C. Homozygous individuals are circled. Scale bar represent 0.5 cm. (D) 40-day old fln2 plants. Seedlings from (C) were transferred to individual pots for continued growth. (E) 70-day old fln2 plants. Plant from (D) after 30 more days of growth. For (D) and (E) bars represent 1 cm.