Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis

Table 1 Tissue-specific FA composition of cotton tissues

	16:0%	16:1%	18:0%	18:1%	18:2%	18:3%	20:0%	MLV%	STC%	DHSA%
root	22.6 ± 0.16	0.4 ± 0.02	16.2 ± 0.43	11.7 ± 0.07	18.9 ± 0.41	10.1 ± 0.32	1.0 ± 0.03	11.9 ± 0.21	6.6 ± 0.13	0.7 ± 0.12
flower	20.7 ± 0.04	1.1 ± 0.01	7.1 ± 0.02	22.7 ± 0.01	20.3 ± 0.06	23.3 ± 0.13	0.8 ± 0.08	3.0 ± 0.02	0.9 ± 0.00	0.1 ± 0.09
seed 0dpa	23.9 ± 0.18	0.5 ± 0.05	11.0 ± 0.42	11.1 ± 0.43	36.5 ± 0.37	16.1 ± 0.34	0.5 ± 0.04		0.2 ± 0.02	0.4 ± 0.06
seed 5dpa	18.3 ± 0.24	0.3 ± 0.02	2.5 ± 0.04	19.6 ± 1.00	31.7 ± 0.37	26.8 ± 0.32	0.3 ± 0.06		0.3 ± 0.02	0.2 ± 0.02
seed 10dpa	20.1 ± 0.19	0.7 ± 0.04	4.8 ± 0.31	15.3 ± 0.72	26.8 ± 0.63	30.7 ± 0.50	0.5 ± 0.03		0.7 ± 0.01	0.4 ± 0.08
seed 25dpa	21.6 ± 0.43	0.6 ± 0.08	3.8 ± 0.21	13.9 ± 0.64	40.7 ± 0.52	18.4 ± 0.33	0.5 ± 0.17		0.3 ± 0.06	0.2 ± 0.06
seed 40dpa	20.3 ± 0.14	0.4 ± 0.02	2.2 ± 0.01	17.8 ± 0.05	53.3 ± 0.14	4.0 ± 0.07	0.4 ± 0.02	0.8 ± 0.01	0.3 ± 0.06	0.4 ± 0.08
seed 50dpa	21.0 ± 0.21	1.0 ± 0.03	2.5 ± 0.35	17.7 ± 0.12	54.4 ± 0.38	1.8 ± 0.25	0.5 ± 0.07	0.5 ± 0.07	0.3 ± 0.14	0.4 ± 0.09

Fatty acid composition from leaf, stem, root, flower and seeds at 0, 5, 10, 25, 40 and 50dpa were analyzed by gas chromatography/mass spectrometry. Results are expressed as a percentage of the total seed fatty acids, and 18:1 is the sum of 18:1Δ⁹ and Δ¹¹ isomers. Values represent the mean and standard deviation of three replicates. MLV: malvalic adid; STC: sterculic acid; DHSA: dihydrosterculic acid.

ISSN: 1471-2229