HRM analysis of the SNP in exon 4 of PpTFL1 using varied level of genomic DNA templates. A. Two levels (7 and 70 ng) of genomic DNA templates of cultivar 16, cultivar 29 and a 1:1 mixture of the two cultivars were used in a primary PCR amplification of exons 3+4. B. Diluted PCR products were used in the second round PCR using nested primers, producing internal amplicons of exon 4 which were analyzed by HRM.