Figure 5
Comparison of HRM of pooled samples after standard PCR or COLD-PCR. A. Standard PCR/HRM analysis of a 131 bp amplicon from PpAG exon 4. Cultivar 30 was examined in pools of six (Y4), twelve (Y4 + Y5), and eighteen (Y4 + Y5 + Y6) genotypes. Percentage of SNP-bearing allele in pool is shown in parentheses. B. COLD-PCR/HRM analysis of a 131 bp amplicon from PpAG exon 4. C. Standard PCR/HRM analysis of a 149 bp amplicon from PpTFL1 exon 4. Cultivar 16 was examined in pools of six (X1), twelve (X1 + X2), and eighteen (X1 + X2 + X3) genotypes. D. COLD-PCR/HRM analysis of a 149 bp amplicon from PpTFL1 exon 4. Each pool was examined in triplicate. Line colors indicate grouping by LC480 Gene Scanning software.