HRM analysis of PpAG exons 4 and 5. A. HRM profile of pooled cultivars. Relative difference plot shows melting changes of pools compared to group X1. Six pools with altered melting profiles have 8 cultivars in common (insert). B. Relative difference plot of cultivars 7, 8 9, 12, 25, 26, 27, and 30. Each cultivar was mixed 1:1 with wild-type cultivar 16 to detect potential homozygous SNPs.