Identification of cultivars containing SNPs in PpTFL1 exon 3 and 4. A. Relative difference plot of cultivars 16, 17, 28, and 29. Each cultivar was mixed 1:1 with wild-type cultivar 6 to detect potential homozygous SNPs. Line colors indicate grouping by LC480 Gene Scanning software. B. Validation of SNPs by sequencing. Wild-type sequence (cultivar 17), homozygous SNP (cultivar 29), and heterozygous SNPs (cultivars 16, 28) at polymorphic site indicated by arrow.