HRM analysis of PpTFL1 exons 3 and 4. A. Two-dimensional pooling of peach cultivars numbered 1-36. Pools of 6 cultivars were designated X1-X6 and Y1-Y6. Four pools (bold) with altered melting profiles have 4 cultivars in common (circled). B. HRM profile of pooled cultivars. Relative difference plot shows melting changes of pooled DNA compared to group X1.