Skip to main content

Table 1 Kinetics of digestion1 of the resistant starch assay for Wt and sbe1a mutant starch2

From: Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination

Starch y 0 (%) S 1 (%) k 1 (min-1) S2(%) k2(min-1)
Wt -5.4 ± 2.3a 85.9 ± 3.5b 1.4 ± 0.1a (×10-2) 17.9 ± 5.4a 0.9 ± 0.2a (×10-3)
sbe1a 13.7 ± 2.8b 59.8 ± 3.0a 1.8 ± 0.1b (×10-2) 24.3 ± 2.4a 3.0 ± 1.1b (×10-3)
  1. 1 Kinetic parameters are obtained from model fit using the double exponential decay equation: where y is % NDS, x is the time, y 0 is the y-value that the model asymptotically approaches, S 1 and S 2 are the concentrations of the two different substrate components, and k 1 and k 2 are the reaction rate constants for the decay of the two different components.
  2. 2 Values are expressed as mean ± SD for three biological replications. Values for each biological replication were obtained from fit of combined data from two independent digestions. Significant differences (p < 0.05) in the same column, as determined by one-way ANOVA analysis, are indicated by different superscripts.