Association of Asr1 with a repressive histone epigenetic mark. ChIP was performed using Dynabeads protein A (Invitrogen) and anti-H3K4me3 or anti-H3K27me3 antibodies (Abcam). Quantitative Real-Time PCR was carried out as indicated in the Methods section. Comparison between non-stress vs. stress yielded p < 0.05. Actin was included as a housekeeping gene control.