Asr1 basal methylation status. Leaf genomic DNA was subjected to the bisulphite procedure and then cloned (9 independent clones) and sequenced. Results are displayed as dot plots (Kismeth software) as described in the Methods section. Filled circles, methylated; empty circles, unmethylated. The numbers indicate cytosine positions beginning from the first analysed cytosine. Residues 32 and 33 are particular cytosine residues that were individually analyzed later (Figure 6.).