Table 1 Effect of the type of target tissue on transformation efficiency
Type of target tissue | No. of co-cultivated calli | No. of transformed calli | Transformation efficiency (%) |
|---|---|---|---|
Embryogenic callus | 154 | 6 | 3.90 ± 5.80 |
Established embryogenic cell suspension (4 months) | 192 | 4 | 0.02 ± 0.03 |
Established embryogenic callus culture (4 months) | 240 | 41 | 17 ± 0.02 |
- *Transformation efficiency was estimated by the proportion (p) of transformed calli (p = x/n), where x is the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula
with a level of confidence of 99%
with a level of confidence of 99%