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Table 1 Effect of the type of target tissue on transformation efficiency

From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

Type of target tissue No. of co-cultivated calli No. of transformed calli Transformation efficiency (%)
Embryogenic callus 154 6 3.90 ± 5.80
Established embryogenic cell suspension (4 months) 192 4 0.02 ± 0.03
Established embryogenic callus culture (4 months) 240 41 17 ± 0.02
  1. *Transformation efficiency was estimated by the proportion (p) of transformed calli (p = x/n), where x is the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula with a level of confidence of 99%