Southern blot analyses of transgenic coffee plants. Coffee DNA from leaf material was digested with EcoRI, electrophoresed and probed with a 32P labeled HPTII gene fragment. The number of bands reflects the number of transgene insertion sites. Lane 1, untransformed coffee (control). Lanes 2-12, transgenic plants from independent transformation events. P - plasmid control (pMDC32). The size of the markers is indicated on the left.