Figure 4From: Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus culturesTransformation efficiency depending on the phenotype of maintained embryogenic callus cultures. Transformation efficiency was assessed by observing GFP epifluorescence 6 weeks after the end of co-cultivation. Transformation efficiency was estimated by the proportion (p) of transformed calli (p = x/n), where x is the number of transformed calli and n the number of co-cultivated calli. A 3 δ confidence limit for binomial distribution was calculated using the formula with a level of confidence of 99%. Confidence interval is indicated by vertical bars. For each callus phenotype, all the transformation experiments were conducted independently in three replicates comprising 40 calli (120 calli/phenotype).Back to article page