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Table 3 List of the spots/proteins identified by mass spectrometry.

From: Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin

GO Spot ID Protein (Species) GB Score % Cv Mw (kD) (Exp/Theo) pI (Exp/The) Pep-tides CPT/CON
Stress responses
  U1 Chloroplast Cu/Zn superoxide dismutase (maize) NP001108127 128 24 17.51/20.99 4.71/5.45 2 CPT
  U2 Cytosolic ascorbate peroxidase (maize) ACG38188 114 11 26.76/27.46 5.35/5.55 3 3.25
  U3 Glutathione S-transferase 19 (maize) AAG34827 78 12 24.60/25.40 6.37/7.68 2 CPT
Defense
  D1 Pathogenesis-related protein 1 (maize) ACG29538 150 34 17.10/17.07 4.98/5.39 3 CON
  D2 Bet v I allergen (maize) AY754698 66 24 18.30/17.07 3.80/4.68 3 CON
Protein degradation
  D3 26S proteasome non-ATPase regulatory subunit 4 (maize) ACG37494 138 6 60.32/42.4 3.54/4.46 2 CON
  U4 26S proteasome non-ATPase regulatory subunit 8 (maize) ACG40420 138 14 29.87/31.05 4.70/5.03 3 CPT
Protein synthesis
  D4 Eukaryotic translation initiation factor eIF-5A (maize) CAA69225 197 70 20.01/17.70 5.38/5.61 7 CON
  D5 Eukaryotic translation initiation factor eIF-6 (maize) ACG33598 92 6 26.93/27.01 3.95/4.64 1 0.46
DNA replication, recombination & repair
  D6 Histone H2B (maize) CAA40564 48 12 19.55/16.41 5.75/10.00 2 CON
RNA metabolism
  D7 RraA (rice) NP916709 101 26 19.01/18.06 5.35/6.12 4 0.5
  U5 RraA (maize) ACG30537 307 39 18.58/18.19 4.95/5.33 6 CPT
  D8 DEAD-family RNA helicase (rice) NP921002 128 30 27.72/26.60 6.46/6.82 5 CON
  U6 Glycine-rich RNA-binding protein 8 (maize) ACG35695 70 8 23.60/21.38 7.00/7.63 2 CPT
  D9 RNA recognition motif containing protein (maize) ACN31194 150 15 32.33/30.23 9.01/8.57 3 0.87
Metabolism
  D10 Cytosolic GAPDH (maize) CAA51676 70 4 20.19/36.63 5.88/6.46 1 CON
  U7 Glyceraldehyde-3-phosphate dehydrogenase (Arabidopsis) AAK15554 74 14 40.95/44.97 7.24/8.75 4 3.23
  D11 Glyceraldehyde-3-phosphate dehydrogenase (Arabidopsis) JQ1287 78 4 20.19/37.07 5.88/6.34 4 CON
  D12 Triosephosphate isomerase 1 (maize) AAB81110 139 40 25.94/27.24 5.34/5.52 8 CON
Signalling
  U8 Protein-G β-subunit (rice) NP916988 100 21 36.34/36.66 5.86/5.97 4 2.84
Endomembrane trafficking
  D13 Syntaxin 6 (rice) NP001065843 121 42 32.32/24.10 6.70/5.56 7 CON
Energy
  U9 Mitochondrial ATP synthase precursor (maize) ACG38121 84 11 29.87/25.57 4.7/7.71 2 CPT
Embryo and seed storage
  U10 Vicilin (rice) CAA41810 86 15 14.12/65.45 5.00/6.63 5 CPT
  U11 Vicilin (rice) CAA41810 132 21 19.48/65.45 4.99/6.63 7 CPT
  U12 Vicilin (rice) CAA41810 110 8 22.12/65.45 4.77/6.63 3 CPT
  U13 Vicilin (rice) CAA41810 75 2 14.2/66.63 9.3/6.23 1 CPT
  U14 globulin 2 (maize) 1802402A 64 3 15.03/50.23 6.03/6.16 1 CPT
  D14 globulin 2 (maize) 1802402A 66 2 17.93/50.23 6.64/6.16 1 CON
  U15 globulin 2 (maize) 1802402A 90 16 21.94/50.23 6.95/6.16 6 2.37
Unknown
  D15 cupin domain containing protein (rice) EAY88907 68 3 20.19/68.6 5.21/5.74 1 CON
  U16 r40c1 protein (rice) ACF87898 71 3 19.6/38.77 6.2/6.3 1 CPT
  1. U, proteins up-regulated by CPT. D, proteins down-regulated by CPT. GB, protein accession number in GenBank. Cv, coverage percentage. Mw/pI, theoretical and experimental molecular weight and isoelectric point. Peptides, tryptic peptides number. Changes in the relative spot volume are represented CPT/CON ratio of the mean from three independent replicas. CPT means presence only in treated samples, and CON presence only in control samples. The proteins were classified according to GO functional categories.