Figure 7

Northern blot analysis of genes encoding proteins identified by comparative proteomic analysis. Northern blot analysis (20 μg of total RNA per sample) showing expression of nine selected genes coding for proteins identified by comparative proteomic analysis in immature maize embryos treated with camptothecin 50 μM: glycine-rich RNA-binding protein 8, EU963577; pathogenesis-related protein 1, AY103811; ascorbate peroxidase 1, NM_001112030; eukaryotic translation initiation factor 5A, AY103556; guanine nucleotide-binding protein beta subunit-like, EU952736; glutathione S-transferase 19, AF244684; 26S proteasome non-ATPase subunit 4, BT018579; putative RNA-binding protein (RRM superfamily), BT065318; triosephosphate isomerase 1, AY103629]. Hybridization of the 18S ribosomal RNA was used as loading control. The corresponding protein spot ID and the relative fold-change (CPT/CON) normalised by 18S rRNA are indicated.