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Table 1 cDNA Library Attributes

From: Identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape (Vitis viniferaL.) based on curation and mining of large-scale EST data sets

Tissue

dbEST Library ID

cDNA Orientation (5'/3')

Submitted Description

Developmental Stage

ESTs as per dbEST

Unique clones

Stressed Leaf

10208

both

An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera cv. Chardonnay

Juvenile & adult

24,400

21,499

Stressed Berry

12435

both

An expressed sequence tag database for abiotic stressed berries of Vitis vinifera cv. Chardonnay

Mixed: 8, 9, 11, 13, 15, 16 weeks DAF

21,384

18,963

Leaf

12752

both

Cabernet Sauvignon Leaf - CA32EN

Mid-season

2,669

1,465

Leaf

12753

both

Cabernet Sauvignon Leaf-CA48EN

Mid-season

2,051

1,104

Leaf

12948

both

Cabernet Sauvignon Leaf - CA48LN

Late Season

2,248

1,441

Leaf

12949

both

Cabernet Sauvignon Leaf - CA41LN

Late Season

1,146

739

Leaf

14446

5'

Grape Leaf pBluescript Library

Juvenile

528

528

    

Leaf subtotal

8642

5277

Berry

4059

both

Grape berries Lambda Zap II Library

Véraison

105

96

Berry

8669

3'

Green Grape berries Lambda Zap II Library

Pre-véraison

1,989

1,989

Berry

8670

3'

Ripening Grape berries Lambda Zap II Library

Post-véraison

3,268

3,267

Berry

8671

both

Véraison Grape berries Lambda Zap II Library

Véraison

96

96

Berry

11063

3'

Véraison Grape berries SuperScriptTM Plasmid Library

Véraison

623

623

Berry

11064

3'

Véraison Grape berries Lambda Zap II Library

Véraison

1,691

1,691

Berry

12754

both

Cabernet Sauvignon Berry - CAB2SG

Pre-véraison

4,429

2,339

Berry

13015

both

Cabernet Sauvignon Berry Stage I - CAB3

Pre-véraison

3,414

1,955

Berry

13016

both

Cabernet Sauvignon Berry - CAB4

Pre-véraison

3,836

2,155

Berry

13017

both

Cabernet Sauvignon Berry Post-Véraison - CAB7

Post-véraison

3,558

1,911

Berry

14444

5'

Grape Berry pSPORT1 Library

Véraison

1,743

1,743

Berry

20043

n.d.

Clusters 4 cm (VvC3)

Pre-véraison

4,053

4,053

Berry

20044

n.d.

Berries Véraison stage (VvC4)

Véraison

3,035

3,035

    

Berry subtotal

31840

24953

Root

14445

5'

Grape Root pSPORT1 Library

One year-old root

1,555

1,555

Root

16696

n.d.

Vitis vinifera Cabernet Sauvignon root

n.d.

102

102

Stressed Root

22274

5'

VVM - Normalized Cabernet Sauvignon root

Young vines

16,452

16,452

Total

    

104,402

88,828

  1. Libraries generated or used in the present study. The Stressed Leaf (SL) and Stressed Berry (SB) libraries were generated previously [6], the Stressed Root "VVM" library was generated specifically for this study, and all other libraries were obtained from the dbEST database maintained by the NCBI. Tissues, dbEST library identifier, sequencing direction, and library descriptions are provided. Unique clones were identified from the ESTs of bidirectionally sequenced libraries as described in the "Methods" and "Results" sections.
  2. n.d. not determined