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Table 4 Daytime expression levels of RBCS1 genes in the leaves of young plants of C. arabica.

From: RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress

   Irrigated (I) Non-Irrigated (NI)
  S - Y RQ Cc RQ Ce RQ RBCS1-T Cc/Ce RQ Cc RQ Ce RQ RBCS1-T Cc/Ce
  U-08 8.70 0.05 11.93 189.44 (*) (*) (*) (*)
I59 (1) WS-08 32.80 0.09 32.66 253.33 21.83 0.13 22.49 167.92
  U-09 22.27 3.28 23.01 6.79 23.69 0.32 23.28 74.05
  WS-09 10.80 0.66 12.33 16.36 3.06 - 3.61  
  U-08 - 15.08 13.95 - (*) (*) (*) (*)
Rubi (1) WS-08 - 14.39 12.15 - - 1.97 2.25 -
  U-09 - 9.78 7.58 - - 5.20 5.65 -
  WS-09 - 13.40 13.27 - - 2.37 2.64 -
Icatú (2) WS-10 13.73 1.14 11.95 12.04 3.69 0.61 3.42 6.09
Rubi (2) WS-10 - 8.95 10.02 - - 1.14 1.15 -
Obatã (2) WS-10    12.19 -    3.60 -
I59 (2) WS-10    26.15 -    17.05 -
  1. Nine-month-old plants (1: at the date of the U-08 point of analysis) grown in field conditions were studied during two consecutive years (2008 and 2009). Twenty-month-old plants (2: at the date of the WS-10 point of analysis) were analysed only during the dry season of 2010. For each cultivar, the season (S) and the year (Y) of analysis are indicated: U (unstressed condition) corresponding to the rainy season and WS (water stress) to the dry season. Corresponding predawn leaf water potentials (Ψpd) are given in Table 3. RBCS1 gene expression was expressed in relative quantification (RQ) for the I59 (IAPAR59), Rubi, Icatú and Obatã cultivars grown in the field with (I) or without (NI) irrigation. RBCS1 targets correspond to CaCe (Ce), CaCc (Cc) and total RBCS1 (RBCS1-T) transcripts amplified by the E18244, C18244 and T18244 primer pairs (Table 1), respectively. Cc/Ce values corresponded to RQ Cc /RQ Ce ratios. For the U-08 point of harvest (*), qPCR analyses were not performed for non-irrigated (NI) plants that were considered identical to irrigated (I) ones. For the Rubi cultivar, CaCc gene expression was not detected (-). In that case, CaCe expression (RQ Ce ) and total RBCS1 gene expression (RQ RBCS1-T ) was deduced from qPCR experiments that used the E18244 and T18244 primer pairs. For Obatã and I59 cultivars analysed in 2010, CaCe (Ce)- and CaCc (Cc)-specific expression was not determined (nd), and RQ RBCS1-T was deduced from qPCR experiments that used the T18244 primer pair. Results were normalised using the expression of the GAPDH reference gene.