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Table 1 List of primers used for gene cloning and quantitative PCR experiments

From: RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress

Gene name

Source gene

Primer name

Primer sequence

bp

UBI *

SGN-U637098

BUBI-F

BUBI-R

5' AAGACAGCTTCAACAGAGTACAGCAT 3'

5' GGCAGGACCTTGGCTGACTATA 3'

104

GAPDH *

SGN-U637469

GAPDH-F

GAPDH-R

5' TTGAAGGGCGGTGCAAA 3'

5' AACATGGGTGCATCCTTGCT 3'

59

RBCS1-Cc (CaCc)

SGN-U617577

FR728242

C18244-F

C18244-R

5' CCGTCCTCTTCCCCTCAAAT 3'

5' CCTGAAAGTACAGCCCCAGTTC 3'

91

RBCS1-Ce (CaCe)

SGN-U607188

AJ419826

E18244-F

E18244-R

5' TTGGCCCCGGCCCCTCAAATT 3'

5' CAGCTAAAAGTACAGCCCCAGTTC 3'

93

RBCS1-T

 

T18244-F

T18244-R

5' CTAGCATGGTTGCACCCTTCA 3'

5' AGTAATGTCGACGGACTTCTTGGA 3'

77

RBCS1-DNA

 

18244-F

18244-R

5' GAGAATGGCATCCTCAATGATCTC 3'

5' CAGCCCCAGTTCTCAATTTTATTG 3'

660(C)

648(E)

  1. Primers were designed using Primer Express software (Applied Biosystems). The source gene indicates the accession numbers of coffee cDNA and gene sequences found in the GenBank and SOL Genomics Network (SGN, http://solgenomics.net/content/coffee.pl[56]) libraries and used to design the primer pairs. The size of the amplicon is indicated in base pairs (bp). E: C. eugenioides corresponding to the CaCe (RBCS1-Ce isoform). C: C. canephora corresponding to the CaCc (RBCS1-Cc isoform). The RBCS1-T primer pair was used to amplify total-RBCS1 (CaCe+CaCc) transcripts. The RBCS1-DNA primer pair was used to amplify the CaCc cDNA and gene sequences. Primer sequences of reference genes previously reported by Barsalobres-Cavallari et al. [101] are also given (*).
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BMC Plant Biology

ISSN: 1471-2229

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