The expression profiles of RBCS1 in C. canephora. For northern blot experiment (A), total RNAs (15 μg) were extracted from leaves of clones 14, 22, 73 and 120 of Conilon grown with (I) or without (NI) irrigation, separated by agarose gel electrophoresis and hybridised independently with CcRBCS1 (RBCS) and CcUBQ10 (UBI) cDNA probes. Total RNA (rRNA) stained with ethidium bromide was used to monitor equal loading of the samples. (B) The qPCR analysis was performed using the C18244 primer pair specific for the CaCc isoform of the RBCS1 genes. Expression levels are indicated in relative quantification of RBCS1 transcripts using the expression of the CcUBQ10 gene as a reference. Results are expressed using 14I as an internal calibrator. In each case, values are the mean of three estimations ± SD. (C) Values of relative quantification (RQ) are given for clones 14, 22, 73 and 120 grown with (I, Ψpd ≈ -0.02 MPa) or without (NI, Ψpd ≈ -3.0 MPa) irrigation. RBCS1 targets correspond to the CaCc gene amplified with the C18244 primer pair. The I/NI ratio of RBCS1-Cc gene expression (Cc I/NI) is also indicated.