Figure 2

Alignment of the RBCS1 genes from C. arabica and C. canephora. The CaRBCS1 gene [GenBank:AJ419827], previously cloned from C. arabica [58], corresponded to the C. eugenioides (CaCe: RBCS1-Ce) allele, while the CcRBCS1 gene [GenBank:FR772689, this work] corresponded to the C. canephora (CaCc: RBCS1-Cc) allele. Horizontal arrows as well as nucleotides are in bold and italics and correspond to primer sequences. The 18244-F and -R primers were used to amplify the CcRBCS1 (Table 1). The RBCS-I1-F1 (RBCS_intron1_F1) and -R1 (RBCS_intron1_R1) primers were used for the mapping of the CcRBCS1 gene [64]. The stars below the alignments indicate identical bases, and the nucleotides are numbered for each lane. A schematic representation of the CaCe and CaCc genes is also given. Exons are boxed and numbers indicate fragment sizes in base pairs.