Table 3 Mutations identified by TILLING in this study
From: TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.)
Treatment:0.4% EMS for 12 hr. | ||||
|---|---|---|---|---|
Gene | Nucleotide Change | Predicted AA Change | Population | Plant ID |
Ara h 2.01 | C145 → T | L49 → F | 05 | 20-6 |
Ara h 2.01 | G164 → A | R55 → H | 05 | 37-4 |
Ara h 2.01 | G192 → A | silent | 05 | 37-4 |
Ara h 2.01 | G186 → A | silent | 07G | 78-4 |
Ara h 2.01 | C80 → T | silent | 07G | 90-4 |
Ara h 2.01 | G357 → A | silent | 07JKEMS1 | 65 |
Ara h 2.01 | G186 → A | silent | 08GH | 250 |
Ara h 2.02 | G185→ A | R62 → Q | 07G | 89-5 |
Ara h 2.02 | G3 → A | disrupted start codon | 08GH | 2 |
Ara h 1.01 | C1392→ T | R333 → W | 07G | 95-1 |
Ara h 1.01 | C586 → T | silent | 07JKEMS1 | 99 |
Ara h 1.02 | C304 → T | R102 → Stop | 07JKEMS1 | 133 |
AhFAD2A | A448 → G | N150 → D | 05 | 4-3 |
AhFAD2A | A448 → G | N150 → D | 05 | 55-4 |
AhFAD2A | A448 → G | N150 → D | 05 | 138-10 |
AhFAD2A | C718 → T | silent | 07G | 113-5 |
AhFAD2A | C761 → T | P254 → L | 07JKEMS1 | 72 |
AhFAD2B | A442 insertion | frameshift | 05 | 69-8 |
AhFAD2B | A442 insertion | frameshift | 07G | 81-4 |
AhFAD2B | C566 → T | silent | 07JKEMS1 | 2 |
Treatment:1.2% EMS for 4.5 hr. | ||||
Gene | Nucleotide Change | Predicted AA Change | Population | Plant ID |
Ara h 2.01 | G243 → A | A82 → T | 06EF | 13-6 |
Ara h 2.01 | G192 → A | silent | 06LREMS1 | 8-4 |
Ara h 2.02 | G208 → A | D70 → N | 06EF | 23-7 |
Ara h 2.02 | G208 → A | D70 → N | 06EF | 26-1 |
Ara h 2.02 | G -315 → A | upstream, probably silent | 06EF | 62-6 |
Ara h 1.01 | C1609 → T | P405 → L | 06EF | 53-3 |
Ara h 1.01 | G1704 → A | E437 → K | 06EF | 56-3 |