Figure 5
From: TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.)
Identification of Ara h 1.02 truncation mutant by CAPS marker analysis. A - Primers 1306/1309 were used to amplify Ara h 1.02 from M3 individuals. PCR products were cut with BslI and then separated on 2% agarose gel. Lane 1: DNA size marker. Lane 2: wild-type control (Tifrunner). Lanes 3-7: individual M3 plants. The 293 bp fragment indicates presence of the mutant allele. The homozygous mutant (Lane 6) lacks the 230 bp fragment. B - Diagram of the amplified fragment of Ara h 1.02. Vertical lines represent BslI cut sites. The cut site denoted in red is eliminated by the mutation.