Figure 3
From: TILLING for allergen reduction and improvement of quality traits in peanut (Arachis hypogaea L.)
IgE binding analysis of seed protein extracts from M 4 generation of Ara h 2.01 mutant lines 20-6 and 37-4. A - Equal amount of total protein from seeds of wild type (Georgia Green; Lane 1), mutant line 37-4 (Lane 2), and mutant line 20-6 (Lane 3) loaded on SDS-PAGE stained with Coomassie blue. B - IgE inmunoblot performed with serum from patients with peanut hypersensitivity (HW, DAM, CM, and NF). Lane numbers are the same as in panel 4A.