Figure 2

Southern blot analysis of Ara h 1 in A. hypogaea cv. Georgia Green. The blot was probed with a full-length genomic fragment of Ara h 1.01, which was PCR-amplified from a plasmid, then labeled with ³²P. Lane 1: Genomic DNA digested with HindIII (no sites within either gene). Lane 2: Genomic DNA digested with EcoRI (one site in each gene). Lane 3: Genomic DNA digested with AseI (two adjacent cut sites in Ara h 1.01 (B-genome), but none in Ara h 1.02 (A-genome)).