Skip to main content

Table 3 Multiplexes of polymorphic loci (with their allele size range in bp) for characterizing the Mediterranean olive tree germplasm *.

From: Genomic profiling of plastid DNA variation in the Mediterranean olive tree

Multiplex PCR

Locus no.

Allele size range (bp)

Multiplex PCR

Locus no.

Allele size range (bp)

A-1 (NED-M13)

46

110-112

B-2 (HEX-M13)

48

158-159

 

1

121-124

 

25

174-177

 

9

135-136

 

36

182-183

 

51

139-146

 

52

191-203

 

22

158-159

 

58

234-236

 

41

169-171

   
   

C-1 (FAM-M13)

21

103-104

A-2 (NED-M13)

17

178-179

 

38

109-111

 

28

182-183

 

31

131-133

 

56

188-190

 

15

137-138

 

53

203-204

 

47

154-157

 

50

227-228

 

59

164-165

 

33

235-236

   
   

C-2 (FAM-M13)

6

173-174

B-1 (HEX-M13)

39

105-106

 

49

181-182

 

27

112-113

 

24

187-189

 

23

120-121

 

29

203-204

 

11

126-136

 

57

224-227

 

42

137-139

 

54

231-239

 

2

148-150

   
  1. * After PCR, the six multiplex PCRs (35 loci) were mixed together with locus 10 (allele size range of 87 to 95 bp) and ROX 500 as internal standard, and then run on an ABI Prism 3100 Genetic Analyzer.