Figure 4

Analysis of transgenic plants transformed with BRI1:bri1-120-GFP. A. Confocal microscopic observations of the GFP signal fused with BRI1(S399F) or intact BRI1 were performed on the root tips. Microscopic features of the same root tissues under bright-field were are shown side-by-side. B. Overexpression of BRI1:bri1-120-GFP in bri1-301 led to an allelic series of the bri1 phenotype. Representative fully rescued (Line 1), intermediate (Line 2), and strong bri1 mutant-looking transgenic bri1-301 plants are shown with un-transformed bri1-301. C. Analysis of BRI1 expression and determination of BRI1 protein amount in transgenic bri1-301 overexpressing BRI1:bri1-120-GFP detected by anti-GFP antibodies and anti-BRI1 antibodies.