Figure 4

Relative accumulation of Dicer-like and Argonautes mRNAs in M. truncatula in different water status. The shoots (green) and roots (brown) of M. truncatula were the organs analyzed in the different water treatment conditions imposed. Values are the mean of two technical replicates of three independent cDNAs for each treatment and bars represent standard errors. The relative mRNA accumulation was calculated using L2 as the reference gene and normalized against the shoot control treatment. The AGO1 and DCL1 primer pair was designed to give one amplicon with the cleavage site of their corresponding miRNA, miR168 and miR162 respectively. A One Way ANOVA Test of significance was used to compare the four conditions in each organ followed by the Tukey Test (p-value <0.05). Ct, Control; MWD, Moderate Water Deficit; SWD, Severe Water Deficit, Rec, Recovery.