Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress

Table 1 Summary of drought responsive SSH libraries and ESTs.

Name of Library	Tester (condition/genotype/tissue)	Driver (Condition/genotype/tissue)	No of clones	Total no of ESTs	HQS	Type of transcripts clones
AS1-1	WS/ICC 4958/Shoot	WW/ICC 4958/Shoot	960	807	753	Up regulated in shoot tissue under drought stress
AS2-1	WW/ICC 4958/Shoot	WS/ICC 4958/Shoot	960	877	821	Down regulated in shoot tissue under drought stress
AR1-1	WS/ICC 4958/Root	WW/ICC 4958/Root	1440	1424	1281	Up regulated in root tissue under drought stress
AR2-1	WW/ICC 4958/Root	WS/ICC 4958/Root	960	940	799	Down regulated in root tissue under drought stress
AB1-1	WS/ICC 4958/Root	WS/ICC 1882/Root	576	576	503	Up regulated in roots of resistant genotype (ICC 4958) under drought stress
AB2-1	WS/ICC 1882/Root	WS/ICC 4958/Root	576	576	529	Down regulated in roots of resistant genotype (ICC 4958) under drought stress
Bulk1-1	WS/Bulk HRB/Root	WS/Bulk LRB/Root	480	423	400	Up regulated in roots of extremes bulks of RILs of HRB under drought stress
Bulk2-1	WS/Bulk LRB/Root	WS/Bulk HRB/Root	480	429	408	Down regulated in roots of extremes bulks of RILs of HRB under drought stress
Total ESTs			6432	6053	5494
Total unigenes					3062

High root biomass genotype (HRB) ICC 4958 and low root biomass genotypes (LRB) ICC 1882 are tagged with "A" and "B" for library description respectively. HQS: High quality sequences, 1-1: Forward subtraction, 2-2: Reverse subtraction, WW: Well watered, WS: Water stressed plants.

ISSN: 1471-2229