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Table 2 Transcriptional profiles of genes in Norton berry skin that are associated with secondary metabolism

From: Berry skin development in Norton grape: Distinct patterns of transcriptional regulation and flavonoid biosynthesis

ClusterA

Affymetrix ChipID

Genoscope ID

Function (VitisNet)B

KEGG

Pathway (VitisNet)

1

VVTU703_s_at

GSVIVT00018175001

Phenylalanine ammonia lyase 2 (PAL2)

4.3.1.5

Phenylpropanoid

1

VVTU12705_s_at

GSVIVT00024561001

Phenylalanine ammonia lyase (PAL)

4.3.1.5

Phenylpropanoid

18

VVTU26285_at

GSVIVT00013936001

Phenylalanine ammonia lyase (PAL)

4.3.1.5

Phenylpropanoid

4

VVTU39693_at

GSVIVT00008924001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

6

VVTU2766_at

GSVIVT00011484001

Sinapyl alcohol dehydrogenase (SAD)

1.1.1.195

Phenylpropanoid

10

VVTU14855_at

GSVIVT00024588001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

20

VVTU21888_at

GSVIVT00011639001

Cinnamyl alcohol dehydrogenase (CAD)

1.1.1.195

Phenylpropanoid

2

VVTU13147_s_at

GSVIVT00013987001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

7

VVTU12930_s_at

GSVIVT00033763001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

12

VVTU3517_at

GSVIVT00015738001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

13

VVTU914_at

GSVIVT00038153001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

20

VVTU15680_at

GSVIVT00020726001

Cinnamoyl-CoA reductase (CCR)

1.2.1.44

Phenylpropanoid

13

VVTU4884_at

GSVIVT00002825001

Caffeoyl-CoA O-methyltransferase (CCoAOMT)

2.1.1.104

Phenylpropanoid

18

VVTU36108_at

GSVIVT00025990001

Caffeic acid O-methyltransferase (CAOMT)

2.1.1.68

Phenylpropanoid

18

VVTU6966_s_at

GSVIVT00026179001

Caffeate 3-O-methyltransferase 1 (COMT)

2.1.1.68

Phenylpropanoid

12

VVTU34546_at

GSVIVT00009234001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU34913_at

GSVIVT00007353001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU34551_x_at

GSVIVT00031875001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU11765_at

GSVIVT00004049001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU7619_x_at

GSVIVT00005196001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU2775_x_at

GSVIVT00007358001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU18886_x_at

GSVIVT00007364001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

18

VVTU6035_x_at

GSVIVT00009221001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

20

VVTU26310_s_at

GSVIVT00031885001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

20

VVTU2671_at

GSVIVT00009225001

Stilbene synthase (STS)

2.3.1.95

Phenylpropanoid

7

VVTU15752_at

GSVIVT00002505001

Pinoresinol forming dirigent protein

DIRPR

Phenylpropanoid

16

VVTU8264_at

GSVIVT00023306001

p-Coumaroyl shikimate 3'-hydroxylase isoform 1

K09754

Phenylpropanoid

14

VVTU25372_at

GSVIVT00017649001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

18

VVTU8974_at

GSVIVT00036840001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

14

VVTU34012_at

GSVIVT00017653001

Ferulate 5-hydroxylase (F5H)

K09755

Phenylpropanoid

2

VVTU6513_s_at

GSVIVT00038750001

Pinoresinol-lariciresinol reductase

PLR

Phenylpropanoid

15

VVTU15529_s_at

GSVIVT00021542001

Secoisolariciresinol dehydrogenase

SIRD

Phenylpropanoid

20

VVTU2645_at

GSVIVT00031383001

4-Coumarate-CoA ligase 2 (4CL)

6.2.1.12

Phenylpropanoid

1

VVTU17924_s_at*

GSVIVT00014584001

Dihydroflavonol 4-reductase (DFR)

1.1.1.219

Flavonoid

12

VVTU14294_at

GSVIVT00036313001

Dihydroflavonol-4-reductase (DFR)

1.1.1.219

Flavonoid

13

VVTU36178_s_at*

GSVIVT00001063001

Leucoanthocyanidin dioxgenase (LDOX)

1.14.11.19

Flavonoid

11

VVTU9714_at

GSVIVT00007249001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

13

VVTU33390_s_at

GSVIVT00031249001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

14

VVTU13981_at

GSVIVT00007247001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

18

VVTU2456_s_at

GSVIVT00015347001

Flavonol synthase (FLS)

1.14.11.23

Flavonoid

10

VVTU16387_at

GSVIVT00015842001

Naringenin,2-oxoglutarate 3-dioxygenase

1.14.11.9

Flavonoid

13

VVTU39787_s_at

GSVIVT00036784001

Flavanone 3-hydroxylase (F3H)

1.14.11.9

Flavonoid

13

VVTU37475_at

GSVIVT00037165001

Flavanone 3-hydroxylase (F3H)

1.14.11.9

Flavonoid

1

VVTU7778_at

GSVIVT00034070001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

4

VVTU6932_at

GSVIVT00016437001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

4

VVTU25410_s_at

GSVIVT00036466001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

7

VVTU6362_at

GSVIVT00017654001

Flavonoid 3-monooxygenase

1.14.13.21

Flavonoid

13

VVTU35884_at

GSVIVT00022300001

Flavonoid 3',5'-hydroxylase (F3'5'H)

1.14.13.88

Flavonoid

10

VVTU13083_at*

GSVIVT00005344001

Anthocyanidin reductase (ANR)

1.3.1.77

Flavonoid

13

VVTU9453_at

GSVIVT00000479001

Quercetin 3-O-methyltransferase 1

2.1.1.76

Flavonoid

1

VVTU39820_s_at

GSVIVT00037967001

Chalcone synthase(CHS)

2.3.1.74

Flavonoid

5

VVTU15193_at

GSVIVT00003466001

UDP-glucose:flavonoid 7-O-glucosyltransferase (UFGT)

2.4.1.237

Flavonoid

14

VVTU22370_at

GSVIVT00033493001

UDP-glucose:flavonoid 7-O-glucosyltransferase (UFGT)

2.4.1.237

Flavonoid

13

VVTU17578_s_at*

GSVIVT00014047001

UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT)

2.4.1.91

Flavonoid

3

VVTU15110_at

GSVIVT00001621001

Flavonol 3-sulfotransferase

2.8.2.25

Flavonoid

1

VVTU3684_s_at

GSVIVT00029440001

Chalcone flavanone isomerase (CHI)

5.5.1.6

Flavonoid

17

VVTU563_at

GSVIVT00020652001

Chalcone isomerase (CHI)

5.5.1.6

Flavonoid

10

VVTU9073_x_at

GSVIVT00009968001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

2.4.1.238

Flavonoid

12

VVTU24324_at

GSVIVT00024127001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

18

VVTU35521_at

GSVIVT00024993001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

19

VVTU15768_at

GSVIVT00037558001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

20

VVTU14014_at

GSVIVT00005849001

Anthocyanidin 3-O-glucosyltransferase

2.4.1.115

Anthocyanin

7

VVTU8698_at

GSVIVT00008206001

Anthocyanidin rhamnosyl-transferase

RHATR

Anthocyanin

8

VVTU10613_at

GSVIVT00026922001

Anthocyanidin rhamnosyl-transferase

RHATR

Anthocyanin

13

VVTU7774_at

GSVIVT00011809001

UDP-rhamnose/rhamnosyltransferase

RHATR

Anthocyanin

5

VVTU8944_x_at

GSVIVT00001860001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

12

VVTU14620_at

GSVIVT00001853001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

16

VVTU15845_at

GSVIVT00001851001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

17

VVTU15902_at

GSVIVT00001859001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

18

VVTU36907_at

GSVIVT00024130001

UDP-glucose: anthocyanidin 5,3-O-glucosyltransferase

RHGT1

Anthocyanin

3

VVTU5076_s_at

GSVIVT00033502001

UDP-glucoronosyl/UDP-glucosyl transferase UGT75C1

UGT75C1

Anthocyanin

15

VVTU38572_at

GSVIVT00025511001

CYP93A1 2-hydroxyisoflavanone synthase

1.14.13.86

Isoflavonoid

13

VVTU2075_at

GSVIVT00019588001

CYP81E1 Isoflavone 2'-hydroxylase

1.14.13.89

Isoflavonoid

20

VVTU22627_at

GSVIVT00019595001

CYP81E1 Isoflavone 2'-hydroxylase

1.14.13.89

Isoflavonoid

4

VVTU3973_at

GSVIVT00026339001

2'-hydroxy isoflavone/dihydroflavonol reductase

1.3.1.45

Isoflavonoid

8

VVTU6973_at

GSVIVT00003030001

Isoflavone methyltransferase

2.1.1.46

Isoflavonoid

  1. A Clusters in bold exhibit steady increase of transcript abundance post véraison; Clusters in italics show decrease of transcript abundance post véraison. Expression profiling of each cluster is shown in Figure 1. B Function annotation and pathway assignment of each gene were based on VitisNet (http://vitis-dormancy.sdstate.org/pathways.cfm). The genes (DFR, LDOX, ANR, UFGT) with asterisk have the same GSVIVT ID and display similar expression profiling as in qPCR.