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Figure 1 | BMC Plant Biology

Figure 1

From: A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

Figure 1

T-DNA cassettes used in this study and representative Arabidopsis plants displaying a range of aberrant and normal growth patterns. Schematic representation of T-DNA cassettes in (A) pART-P450-cSUC2-BAR, (B) pART-cSUC2-BAR, and (C) pART-uidA-BAR. LB: T-DNA left border; RB: T-DNA right border; P nos -nptII-pA nos : nopaline synthase promoter - neomycin phosphotransferase cDNA - nopaline synthase poly-adenylation signal; P 35S : Cauliflower Mosaic Virus 35S promoter; frt: FLP recombinase recognition target sites; P SSU -P450 SU1 -pA SSU : Rubisco small subunit promoter - P450 SU1 gene encoding CYP105A1 cytochrome P450 monooxygenase - Rubisco small subunit poly-adenylation signal; P SUC2 -cSUC2-pA nos : 2 kb of AtSUC2 promoter - excisable cDNA of AtSUC2 - nopaline synthase poly-adenylation signal; bar-pA nos : Basta (glufosinate ammonium) resistance cDNA - nopaline synthase poly-adenylation signal. Representative 21-day old rosettes of (D) transgenic line OCP-1 (Overexpressing Cytochrome P 450 SU1 ) harboring pART-P450-cSUC2-BAR and displaying a severe phenotype, (E) transgenic line cSUC2-1 harboring pART-cSUC2-BAR, and (F) wild type Arabidopsis. (G) Representative 35-day old OCP-17, OCP-9 (both displaying severe phenotypes), OCP-2 (displaying a moderate phenotype), wild type, and cSUC2-1, as indicated. (H, inset) Representative 50-day old OCP-1 plant showing anthocyanin accumulation and 'twirled' rosette. Scale bar in D - H is 1 cm.

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