Differential disease resistance response in the barley necrotic mutant nec1

BMC Plant Biology

Table 1 Quantitative real-time PCR primer sequences used in the study

Primer	Sequence	Reference
HvBI_cw1	CGATGATCTCCTGCGTGTCG	This study *
HvBI_ccw1	TACCTCGGTGGCCTGCTCTC	This study *
HvGAPDH_cw1	CGTTCATCACCACCGACTAC	[67]
HvGAPDH_ccw1	CAGCCTTGTCCTTGTCAGTG	[67]
MLO_F1	GTCGAGCCCAGCAACAAGTTCTTC	This study *
MLO_R1	ACCACCACCTTCATGATGCTCAG	This study *
HvrbohA_F1	CCGATCAGATGTATGCTCCA	[33]
HvrbohA_R1	CAGAAGGCATTGAAGCCAGT	[33]
HvRACB_L01	GGTAGACAAAGAACAAGGGCGAAGT	This study *
HvRACB_R01	CACAAGGCAGGAAGAAGAGAAATCA	This study *

* Primers were designed using Primer 3 software [68] using the following gene sequences as a template: HvBI (HarvEST21 Unigene 3323; AJ290421); MLO (HarvEST21 Unigene 6351; Z83834); HvRacB (HarvEST21 Unigene 5202; AJ344223)

ISSN: 1471-2229