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Table 4 Time-course of main chloroplast parameters putatively involved in the regulation of gene expression, as previously reported [32, 66, 67].

From: Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein

 

WT

npq1lut2

Time (hours)

0

2

24

48

72

144

0

2

24

48

72

144

qP

1

0,20 ± 0,06

0,15 ± 0,07

0,03 ± 0,02

0,05 ± 0,01

0,10 ± 0,08

1

0,07 ± 0,03

0,08 ± 0,05

0,02 ± 0,01

0,07 ± 0,08

0,13 ± 0,08

Fv/Fm

0,79 ± 0,01

0,48 ± 0,07

0,42 ± 0,03

0,47 ± 0,07

0,43 ± 0,17

0,49 ± 0,07

0,79 ± 0,01

0,51 ± 0,13

0,22 ± 0,10

0,07 ± 0,03 *

0,45 ± 0,09

0,46 ± 0,13

ADP/ATP

2,2 ± 0,2

1,8 ± 0,1

2,2 ± 0,9

2,2 ± 0,6

2,1 ± 0,3

2,3 ± 0,4

2,1 ± 0,2

1,7 ± 0,1

2,5 ± 0,6

2,4 ± 0,1

1,8 ± 0,1

2,3 ± 0,1

GSH/(GSH+GSSH)

91,3 ± 9,5

96,2 ± 14,5

96,3 ± 7,1

95,1 ± 8,2

93,2 ± 10,4

85,7 ± 5,2

96,9 ± 8,5

92,1 ± 7,5

96,6 ± 3,0

91,6 ± 6,1

79,5 ± 20,4

78,5 ± 10,3

Asc/(Asc+DHA)

74,5 ± 4,1

73,1 ± 1,2

78,6 ± 2,4

75,6 ± 2,2

68,9 ± 4,0

71,2 ± 5,3

69,1 ± 2,2

67,8 ± 4,4

74,5 ± 3,4

74,2 ± 2,8

72,9 ± 2,9

53,2 ± 4,0 *

  1. WT and npq1lut2 rosettes were pre-treated for 48 hrs at 10°C (see methods for details), then were exposed to photoxidative conditions (1000 μmol photon m-2 s-1, 10°C, 16 h light/8 h dark). Leaves were harvested, then used for analysis of chlorophyll fluorescence parameters or immediately frozen in liquid nitrogen for measurements of metabolites, at the same time of the day over a 6-day-long stress period. Abbreviations: qP, photochemical quenching; Fv/Fm, maximal PSII photochemical efficiency; GSH/GSSG, glutathione reduced/oxidized; Asc, ascorbate; DHA, dehydroascorbate. Values that differ significantly between wild type and npq1lut2 mutant plants (Student's t-test, p < 0.02) are marked by an asterisk.