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Figure 5 | BMC Plant Biology

Figure 5

From: Molecular, genetic and transcriptional evidence for a role of VvAGL11 in stenospermocarpic seedlessness in grapevine

Figure 5

VvAGL11 transcript profile is genotype dependent at key stages of seed development. The candidate gene VvAGL11 is expressed preferentially at pea size berry development stage and in seeded genotypes ("bb" and "bc"). Homozygous genotypes for the seedless allele ("aa") have a basal expression level, and as expected, heterozygous genotypes ("ab") have an intermediate level of expression. Candidate gene transcript relative abundance was quantified by qPCR along three key stages of seed and berry development in four genotypes differing on their degree of seed development (Table 2). Development stages are pre-bloom (light blue bars), bloom (orange bars) and pea size berries (light green bars). Genotypes for qPCR analysis were chosen among the experimental progeny RS × S based on their genotype defined by intragenic marker VMC7F2 that has a 1:2:1 (ab × ab) segregation where "a" and "b" stand for the seedless and seeded allele respectively. Additionally Red Globe, a seeded table grape variety, was also included ("bc" genotype). Each bar of the analysis represents the average expression between biological replicates. The expression of VvAGL11 was normalized towards EF1-α in the corresponding samples and the results are presented as percentage of the highest value of relative abundance.

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