Figure 2

Molecular analysis and polyamine content of the transgenic plants. PCR amplification of transgenic lines that are transferred to soil pots via specific primers of CaMV35S-MdSPDS1 (A) and NPTII (B). (C) Semi-quantitative RT-PCR analysis on the expression level of MdSPDS1 in the wild type (WT) and two transgenic lines (TG4 and TG9). (D-E) Analysis of free (D) and conjugated (E) polyamine content by HPLC in fully expanded leaves sampled from the WT and transgenic plants grown under the same conditions. *, ** and *** indicate the values are significantly different compared with WT at significance level of P < 0.05, P < 0.01 and P < 0.001, respectively.