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Figure 9 | BMC Plant Biology

Figure 9

From: Glutathione synthesis is essential for pollen germination in vitro

Figure 9

Transmission electron micrographs showing the subcellular distribution of glutathione in pollen grains of pad2-1. Gold particles bound to glutathione could be found evenly distributed in plastids (P), mitochondria (M), and the cytosol but not in lipid bodies (L), vacuoles (V) and cell walls in pollen obtained from the Arabidopsis thaliana mutant pad2-1. Pollen grains were grown on solidified pollen germination medium for 5 hours with either (a) no GSH and BSO (control), (b) 0.1 mM BSO, (c) 1 mM GSH, (d) 0.1 mM BSO and 1 mM GSH, (e) 3 mM GSH, and (f) 3 mM GSH and 0.1 mM BSO prior to fixation. Bars = 1 μm.

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