Validation of gma-miR166 targets matched by identical reads. The numbers of signatures along the sequences of targets were plotted. Red arrows indicate signatures produced by miRNA-directed cleavage. Black arrows above mRNA of targets indicate detected cleavage sites. Red numbers above the black arrows indicate cleavage probabilities (cleaved target vs total sequenced clones) through 5' RACE confirmation. Black numbers on the right or left side of each black arrow indicate detection abundance of reads. (a) Target cleavage signature, cleavage site in HD-ZIP transcription factor gene Glyma15g13640, and confirmation by RLM-5'RACE. (b) Target cleavage features in HD-ZIP transcription factor gene Glyma6g09100 and confirmation by RLM-5'RACE. (c) Cleavage features in HD-ZIP transcription factor gene Glyma08g21610 and confirmation by RLM-5'RACE. For (a), (b) and (c), only one of the two identified cleavage sites was further confirmed by RLM-5'RACE. (d) Gma-miR166 target HD-ZIP transcription factor gene (Glyma07g01950) from degradome sequencing could not be further confirmed by 5' RACE.