Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL

BMC Plant Biology

Table 5 Estimation of relative amounts of sesquiterpene synthases and squalene synthase in various tissues of Artemisia annua using the 2^-ΔΔCTmethod [42]

	Normalized transcript amount relative to *GAS*2^-ΔΔCT
Enzyme	Flower buds	Young leaves	Old leaves	Stems
ADS	8780	6654	69	18
CPS	360	170	24	15
GAS	56	890	11	1
ECS	315	590	43000	34
SQS	830	340	1780	550

The C_T-values in Table 2 were used for the calculations, and β-actin was used as reference gene. The GAS gene in stems was used for the normalization of values.

ISSN: 1471-2229