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Table 2 Average measured CT-values

From: Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL

Transcript*

Flower buds

Young leaves

Old leaves

Stems

Roots

Hairy roots

β-actin

23.8

22.3

23.4

21.6

22.9

21.1

β-actin #

20.8

22.1

22.5

20.7

24.4

20.3

ADS

23.3

22.2

29.9

30.0

n.d.

35.9

ALDH1

25.8

27.7

31.7

34.3

37.4

39,5

CPR

21.5

21.0

19.9

19.5

20.7

21.0

CPS

27.9

27.5

31.4

30.3

35.5

37,7

CYP71AV1

26.9

24.2

33.8

30.6

31.3

30.7

DBR2#

19.9

20.1

27.2

26.4

27.9

29.6

DBR2+OPR3

23.8

23.0

24.4

23.0

23.8

25.1

DXR

24.6

24.7

24.8

23.8

25.6

23.4

DXS

21.9

20.8

23.0

21.1

22.6

23.1

ECS

28.1

25.7

20.6

29.1

30.5

35.6

FDS1/FDS2

24.6

23.5

23.5

23.8

25.3

23.5

GAS

30.6

25.1

32.6

34.2

32.6

34.0

HDR

24.4

22.4

19.7

22.4

25.4

24.3

HMGR

24.6

24.4

28.6

24.6

23.8

24.7

PAL

21.8

20.0

21.3

19.4

20.8

21.7

RED1

31.7

30.7

26.0

34.6

25.7

20.9

SQS

26.7

26.5

25.2

25.1

26.5

25.7

  1. Two independent triplet reactions were used for the calculation of average CT-values. n.d. = not detected. The rows in bold represent the reference samples. The transcripts labelled with # were analyzed in a separate experiment.
  2. * ADS, amorpha-4,11-diene synthase; ALDH1, aldehyde dehydrogenase 1; CPR, cytochrome P450 reductase; CPS, β-caryophyllene synthase; CYP71AV1, amorphadiene-12-hydroxylase; DBR2, artemisinic aldehyde Δ11(13) reductase; DXR, 1-deoxy-D-xylulose 5-phosphate reductase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; ECS, epi-cedrol synthase; FDS, farnesyl diphosphate synthase; GAS, germacrene A synthase; HDR, hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase; HMGR, 3-hydroxy-3-methyl-glutaryl-CoA reductase; OPR3, 12-oxophytodienoate reductase; PAL, phenylalanine ammonia lyase; RED1, dihydroartemisinic aldehyde reductase; SQS, squalene synthase.