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Table 1 Efficiency and nucleotide sequences of primers used in qPCR

From: Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL

Transcript*

Calculated primer efficiency

Forward Primer Sequence

Reverse Primer Sequence

Fragment size (bp)

β-actin

1.91

5'-CCAGGCTGTTCAGTCTCTGTAT-3'

5'-CGCTCGGTAAGGATCTTCATCA-3'

180

ADS

1.88

5'-GGGAGATCAGTTTCTCATCTATGAA-3'

5'-CTTTTAGTAGTTGCCGCACTTCTT-3'

95

ALDH1

1.82

5'-CATCGGAGTAGTTGGTCACAT-3'

5'-GTTTCTGACCCAAATCCAGGTTGA-3'

120

CPR

1.90

5'-GCTCGGAACAGCCATCTTATTCTT-3'

5'-GAAGCCTTCTGAGTCATCTTGTGT-3'

174

CPS

1.85

5'-CAACGATGTAGAAGGCTTGCTTGA-3'

5'-GTAGATAGTGTTGGGTTGGTGTGA-3'

150

CYP71AV1

1.83

5'-CGAGACTTTAACTGGTGAGATTGT-3'

5'-CGAAGCGACTGAAATGACTTTACT-3'

144

DBR2

1.91

5'-GCGGTGGTTACACTAGAGAACTT-3'

5'-ATAATCAAAACTAGAGGAGTGACCC-3'

228

DBR2/OPR3

1.84

5'-ATCATCAACAAGCAAGCCCATTTCAAA-3'

5'-CGATAGTCTTCAACCACCTCTAGTA-3'

125

DXR

1.92

5'-GGTGATGAAGGTGTTGTTGAGGTT-3'

5'-AGGGACCGCCAGCAATTAAGGT-3'

160

DXS

1.92

5'-GTGCTTCCAGACCGTTACATTGA-3'

5'-AGCCTCTCGTGTTTGCCCAAGGT-3'

120

ECS

1.86

5'-GCAACAAGCCTACGAATCACTCAA-3'

5'-CGTGAAAAATTAAGGACCCTCATAG-3'

126

FDS1/FDS2

1.93

5'-ATCTGCCCTTGGTTGGTGTATTGA-3'

5'-GTTGCCCTCTGCGTGTATGAGA-3'

92

GAS

1.83

5'-CTCGTTACTCCTTGGCAAGAATCAT-3'

5'-GCTCCATAGCACTAATATCCCACTT-3'

147

HDR

1.86

5'-TCAGGAGCGACAAGATGCTATGTA-3'

5'-AGTGTGAGGTGTTGCTTGAGTTGA-3'

95

HMGR

1.83

5'-GGGCGTGGAAAATCTGTTGTGTTC-3'

5'-GAACCAGCAATAGCAGAACCAGTAA-3'

136

PAL

1.87

5'-ATCGGGAAACTCATGTTCGCTCAA-3'

5'-AACTTGGGTTACGTCCACCAGAAA-3'

97

RED1

1.92

5'-TGTCAACTGTGTCCATCCAGGTT-3'

5'-ACCATCATCGGGCAACAAAGCAA-3'

118

SQS

1.82

5'-GACCAGTTCCACCATGTTTCTACT-3'

5'-GCTTTGACAACCCTATTCCAACAAG-3'

190

  1. The mean efficiency of the amplicons was calculated by the program Linreg v. 12.1 based on the log linear phase of the amplification curve. The transcripts in bold were used as reference genes in the calculation of expression ratios.
  2. * ADS, amorpha-4,11-diene synthase; ALDH1, aldehyde dehydrogenase 1; CPR, cytochrome P450 reductase; CPS, β-caryophyllene synthase; CYP71AV1, amorphadiene-12-hydroxylase; DBR2, artemisinic aldehyde Δ11(13) reductase; DXR, 1-deoxy-D-xylulose 5-phosphate reductase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; ECS, epi-cedrol synthase; FDS, farnesyl diphosphate synthase; GAS, germacrene A synthase; HDR, hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase; HMGR, 3-hydroxy-3-methyl-glutaryl-CoA reductase; OPR3, 12-oxophytodienoate reductase; PAL, phenylalanine ammonia lyase; RED1, dihydroartemisinic aldehyde reductase; SQS, squalene synthase.