Table 2 Product profiles of recombinant TPS enzymes based upon total ion current of GCMS analysis on a DB-WAX column
TPS | Clone ID | Products* | Percent total |
|---|---|---|---|
MONOTERPENE SYNTHASES | |||
Pg×eTPS-Car1 | WS0063_F08 | (+)-3-Carene | 53.7 |
Terpinolene | 17.2 | ||
(+)-Sabinene | 5.6 | ||
Terpinen-4-ol | 5.2 | ||
(-)-α-Pinene | 2.7 | ||
α-Terpineol | 2.6 | ||
(-)-β-Phellandrene | 2.3 | ||
Myrcene | 2.2 | ||
γ-Terpinene | 0.9 | ||
α-Terpinene | 0.6 | ||
α-Phellandrene | 0.3 | ||
α-Thujene | 0.2 | ||
Others | 6.5 | ||
PsTPS-Car1 | WS02910_I02 | (+)-3-Carene | 66.4 |
Terpinolene | 16.3 | ||
(+)-Sabinene | 4.7 | ||
(-)-α-Pinene | 2.7 | ||
Terpinen-4-ol | 2.5 | ||
(-)-β-Phellandrene | 2.1 | ||
Myrcene | 2.1 | ||
α-Terpineol | 1.4 | ||
γ-Terpinene | 0.8 | ||
Others | 1.1 | ||
PgTPS-Cin | WS02628_N22 | 1,8-Cineole | 89.1 |
(-)-α-Terpineol | 4.7 | ||
(+)-α-Pinene | 1.9 | ||
β-Pinene | 1.9 | ||
Unknown | 1.4 | ||
Myrcene | 1.1 | ||
Pg×eTPS-Cin | WS00921_D15 | 1,8-Cineole | 65.6 |
(-)-α-Terpineol | 18.3 | ||
Myrcene | 4.1 | ||
(+)-α-Pinene | 3.0 | ||
β-Pinene | 2.6 | ||
γ-Terpinene | 1.8 | ||
Others | 4.6 | ||
PsTPS-Cin | WS0291_H24 | 1,8-Cineole | 59.0 |
(-)-α-Terpineol | 12.2 | ||
Myrcene | 9.0 | ||
β-Pinene | 5.5 | ||
(+)-α-Pinene | 4.7 | ||
Others | 9.5 | ||
PgTPS-Lin | WS0054_P01 | (-)-Linalool | 100 |
PsTPS-Lin-1 | WS0285_L07 | (-)-Linalool | 100 |
PsTPS-Lin-2 | WS02915_K02 | (-)-Linalool | 100 |
PsTPS-Phel-1 | WS02729_A23 | (-)-β-Phellandrene | 61.9 |
(-)-β-Pinene | 18.6 | ||
(-)-α-Pinene | 12.3 | ||
Myrcene | 5.4 | ||
α-Phellandrene | 1.0 | ||
α-Terpinolene | 0.5 | ||
PsTPS-Phel-2 | WS0296_I22 | (-)-β-Phellandrene | 61.2 |
(-)-β-Pinene | 19.8 | ||
(-)-α-Pinene | 12.1 | ||
Myrcene | 5.5 | ||
α-Phellandrene | 0.9 | ||
α-Terpinolene | 0.5 | ||
PsTPS-Phel-3 | WS0276_M12 | (-)-β-Phellandrene | 60.9 |
(-)-β-Pinene | 20.9 | ||
(-)-α-Pinene | 12.5 | ||
Myrcene | 4.1 | ||
α-Phellandrene | 1.3 | ||
α-Terpinolene | 0.2 | ||
PsTPS-Phel-4 | WS01042_E12 | (-)-β-Phellandrene | 61.9 |
(-)-β-Pinene | 19.6 | ||
(-)-α-Pinene | 11.5 | ||
Myrcene | 5.2 | ||
α-Phellandrene | 1.2 | ||
α-Terpinolene | 0.6 | ||
PgTPS-Pin-1 | WS00725_G07c1 | (-)-α-Pinene | 66.7 |
(-)-β-Pinene | 33.3 | ||
PgTPS-Pin-2 | WS00725_G07c2 | (-)-β-Pinene | 70.5 |
(-)-α-Pinene | 29.5 | ||
PsTPS-Pin | WS0291_K15 | (-)-α-Pinene | 83.4 |
(-)-β-Pinene | 12.6 | ||
Linalool | 2.1 | ||
β-Phellandrene | 1.0 | ||
Camphene | 0.4 | ||
Myrcene | 0.4 | ||
SESQUITERPENE SYNTHASES | |||
Pg×eTPS-Far/Oci | WS00926_E08 | (E,E)-α-Farnesene/(E)-β-ocimene | 100 |
PgTPS-Hum | WS0074_O16 | α-Humulene | 42.7 |
(E)-β-Caryophyllene | 37.9 | ||
α-Longipinene | 7.5 | ||
Longifolene | 3.1 | ||
α-Muurolene | 2.7 | ||
γ-Himachalene | 2.6 | ||
Others | 3.4 | ||
Pg×eTPS-Lonf | WS00927_M20 | Longifolene | 69.5 |
α-Longipinene | 30.5 | ||
PsTPS-Lonp | WS02712_A08 | α-Longipinene | 47.7 |
Longifolene | 19.9 | ||
γ-Himachalene | 15.9 | ||
(E)-β-Farnesene | 7.0 | ||
β-Longipinene | 3.0 | ||
Others | 6.4 | ||
DITERPENE SYNTHASES | |||
PsTPS-Iso | pSW06061903 | Isopimaradiene | 98.3 |
Sandaracopimaradiene | 1.7 | ||
PsTPS-LAS | WS0299_C21 | Abietadiene | 49.4 |
Levopimaradiene | 23.8 | ||
Neoabietadiene | 23.3 | ||
Palustradiene | 3.5 | ||