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Table 2 Product profiles of recombinant TPS enzymes based upon total ion current of GCMS analysis on a DB-WAX column

From: Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Piceaspp.)

TPS Clone ID Products* Percent total
MONOTERPENE SYNTHASES
Pg×eTPS-Car1 WS0063_F08 (+)-3-Carene 53.7
   Terpinolene 17.2
   (+)-Sabinene 5.6
   Terpinen-4-ol 5.2
   (-)-α-Pinene 2.7
   α-Terpineol 2.6
   (-)-β-Phellandrene 2.3
   Myrcene 2.2
   γ-Terpinene 0.9
   α-Terpinene 0.6
   α-Phellandrene 0.3
   α-Thujene 0.2
   Others 6.5
PsTPS-Car1 WS02910_I02 (+)-3-Carene 66.4
   Terpinolene 16.3
   (+)-Sabinene 4.7
   (-)-α-Pinene 2.7
   Terpinen-4-ol 2.5
   (-)-β-Phellandrene 2.1
   Myrcene 2.1
   α-Terpineol 1.4
   γ-Terpinene 0.8
   Others 1.1
PgTPS-Cin WS02628_N22 1,8-Cineole 89.1
   (-)-α-Terpineol 4.7
   (+)-α-Pinene 1.9
   β-Pinene 1.9
   Unknown 1.4
   Myrcene 1.1
Pg×eTPS-Cin WS00921_D15 1,8-Cineole 65.6
   (-)-α-Terpineol 18.3
   Myrcene 4.1
   (+)-α-Pinene 3.0
   β-Pinene 2.6
   γ-Terpinene 1.8
   Others 4.6
PsTPS-Cin WS0291_H24 1,8-Cineole 59.0
   (-)-α-Terpineol 12.2
   Myrcene 9.0
   β-Pinene 5.5
   (+)-α-Pinene 4.7
   Others 9.5
PgTPS-Lin WS0054_P01 (-)-Linalool 100
PsTPS-Lin-1 WS0285_L07 (-)-Linalool 100
PsTPS-Lin-2 WS02915_K02 (-)-Linalool 100
PsTPS-Phel-1 WS02729_A23 (-)-β-Phellandrene 61.9
   (-)-β-Pinene 18.6
   (-)-α-Pinene 12.3
   Myrcene 5.4
   α-Phellandrene 1.0
   α-Terpinolene 0.5
PsTPS-Phel-2 WS0296_I22 (-)-β-Phellandrene 61.2
   (-)-β-Pinene 19.8
   (-)-α-Pinene 12.1
   Myrcene 5.5
   α-Phellandrene 0.9
   α-Terpinolene 0.5
PsTPS-Phel-3 WS0276_M12 (-)-β-Phellandrene 60.9
   (-)-β-Pinene 20.9
   (-)-α-Pinene 12.5
   Myrcene 4.1
   α-Phellandrene 1.3
   α-Terpinolene 0.2
PsTPS-Phel-4 WS01042_E12 (-)-β-Phellandrene 61.9
   (-)-β-Pinene 19.6
   (-)-α-Pinene 11.5
   Myrcene 5.2
   α-Phellandrene 1.2
   α-Terpinolene 0.6
PgTPS-Pin-1 WS00725_G07c1 (-)-α-Pinene 66.7
   (-)-β-Pinene 33.3
PgTPS-Pin-2 WS00725_G07c2 (-)-β-Pinene 70.5
   (-)-α-Pinene 29.5
PsTPS-Pin WS0291_K15 (-)-α-Pinene 83.4
   (-)-β-Pinene 12.6
   Linalool 2.1
   β-Phellandrene 1.0
   Camphene 0.4
   Myrcene 0.4
SESQUITERPENE SYNTHASES
Pg×eTPS-Far/Oci WS00926_E08 (E,E)-α-Farnesene/(E)-β-ocimene 100
PgTPS-Hum WS0074_O16 α-Humulene 42.7
   (E)-β-Caryophyllene 37.9
   α-Longipinene 7.5
   Longifolene 3.1
   α-Muurolene 2.7
   γ-Himachalene 2.6
   Others 3.4
Pg×eTPS-Lonf WS00927_M20 Longifolene 69.5
   α-Longipinene 30.5
PsTPS-Lonp WS02712_A08 α-Longipinene 47.7
   Longifolene 19.9
   γ-Himachalene 15.9
   (E)-β-Farnesene 7.0
   β-Longipinene 3.0
   Others 6.4
DITERPENE SYNTHASES
PsTPS-Iso pSW06061903 Isopimaradiene 98.3
   Sandaracopimaradiene 1.7
PsTPS-LAS WS0299_C21 Abietadiene 49.4
   Levopimaradiene 23.8
   Neoabietadiene 23.3
   Palustradiene 3.5
  1. Compounds were identified by comparison of mass spectra and retention indices with authentic standards if available, and retention indices, and/or mass spectra from Adams [52] and NIST, and combined mass spectra and retention index library searches in MassFinder [53] if standards were not available.